慶大霉素(Gen)ELISA快速檢測試劑盒（檢組織/肝臟/乳制品，靈敏度0.05ppb，一步法）

慶大霉素(Gen)ELISA快速檢測試劑盒

一、原理

本試劑盒采用間接競爭ELISA方法，在微孔條上預包被偶聯抗原，樣本中的殘留物慶大霉素將和微孔條上預包被的偶聯抗原競爭抗慶大霉素抗體，加入酶標記物后，用TMB底物顯色，樣本吸光值與其所含殘留物慶大霉素的含量成負相關，與標準曲線比較即可得出相應殘留物慶大霉素的含量。

二、試劑盒特性

u  試劑盒靈敏度：       0.05ppb

u  孵育溫度    ：       25℃

u  孵育時間    ：       30min～15min

u  批內、批間差：       <15%

u  樣本檢測下限

組織  ·····································  2.5 ppb

肝臟  ·····································  3 ppb

牛奶  ·····································  3 ppb

u  交叉反應率

慶大霉素   ······································    100%

鏈霉素       ·······································    <1%

雙氫鏈霉素 ········································· <1%

新霉素       ···········································<1%

u  樣本回收率

組織    ·········································  90%±20%

肝臟    ·········································  80%±20%

牛奶  ···········································   90%±18%

三、試劑盒組成

四、樣品前處理步驟及檢測分析步驟

  詳見產品說明書。

五、儲藏條件和保質期

1、 儲藏條件：試劑盒于2～8℃保存，不要冷凍。

2、 保 質 期：該產品有效期為1年，生產日期見包裝盒。

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