鏈霉素(Strep)ELISA檢測試劑盒（檢雞肉組織/牛奶/蜂蜜，靈敏度0.1ppb,兩步法）
鏈霉素(Strep)ELISA快速檢測試劑盒
一、原理
本試劑盒采用間接競爭ELISA方法，在微孔條上預包被偶聯抗原，樣本中的殘留物鏈霉素將和微孔條上預包被的偶聯抗原競爭抗鏈霉素抗體，加入酶標記物后，用TMB底物顯色，樣本吸光值與其所含殘留物鏈霉素的含量成負相關，與標準曲線比較即可得出相應殘留物鏈霉素的含量。
二、試劑盒特性
u 試劑盒靈敏度： 0.1ppb
u 孵育溫度 ： 37℃
u 孵育時間 ： 30min～30min～15min
批內、批間差： <10%
u 樣本檢測下限
雞 肉 ······································ 1 ppb
雞肝、牛奶 ······································ 4 ppb
蜂 蜜/蜂王漿 ······································ 2 ppb
u 交叉反應率
鏈霉素 ············································· 100%
慶大霉素 ············································ ﹤0.1%
雙氫鏈霉素 ··········································· 108%
卡那霉素 ············································ ﹤0.1%
u 樣本回收率
牛 奶 ············································ 85±22%
雞肉組織 ············································ 80±22%
蜂蜜/蜂王漿 ·········································· 75±22%
三、試劑盒組成
1 | 微量測試孔 | 每條8孔，一板12條 | |
2 | 標準液×6瓶 （1ml/瓶） | 0ppb | 0.1ppb |
0.4ppb | 1.6ppb | ||
6.4ppb | 25.6ppb | ||
3 | 酶標記物 | 12ml | 紅色帽 |
4 | 抗體工作液 | 7ml | 藍色帽 |
5 | 底物A液 | 7ml | 白色帽 |
6 | 底物B液 | 7ml | 黑色帽 |
7 | 終止液 | 7ml | 黃色帽 |
8 | 20X濃縮洗滌液 | 40ml | 白色帽 |
9 | 10X濃縮復溶液 | 50ml | 透明帽 |
四、樣品前處理步驟及檢測分析步驟
詳見產品說明書。
五、儲藏條件和保質期
1、 儲藏條件：試劑盒于2～8℃保存，不要冷凍。
2、 保 質 期：該產品有效期為1年，生產日期見包裝盒。
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